Construct: shRNA TRCN0000069026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009785.1-1796s1c1
DNA Barcode:
CGGGATGATGTGTTACGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacna2d3 (12294)
Gene Description:
calcium channel, voltage-dependent, alpha2/delta subunit 3
Transcript:
RefSeq NM_009785.1 (CURRENT)
Match location:
Position 1796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATGATGTGTTACGAAAT
Hairpin Sequence:
5'-CCGG-CGGGATGATGTGTTACGAAAT-CTCGAG-ATTTCGTAACACATCATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATGATGTGTTACGAAATCTCGAGATTTCGTAACACATCATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATGATGTGTTACGAAATCTCGAGATTTCGTAACACATCATCCCG-3'

Other clones with same target sequence:

(none)