Construct: shRNA TRCN0000069147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_483973.1-681s1c1
DNA Barcode:
CCAAACATGGTCAGGGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC544750 (544750)
Gene Description:
similar to Potassium voltage-gated channel subfamily C member 2 (Voltage-gated potassium channel subunit Kv3.2) (KSHIIIA)
Transcript:
RefSeq XM_483973.1 (NON-CURRENT)
Match location:
Position 681 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACATGGTCAGGGATGTT
Hairpin Sequence:
5'-CCGG-CCAAACATGGTCAGGGATGTT-CTCGAG-AACATCCCTGACCATGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACATGGTCAGGGATGTTCTCGAGAACATCCCTGACCATGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACATGGTCAGGGATGTTCTCGAGAACATCCCTGACCATGTTTGG-3'

Other clones with same target sequence:

(none)