Construct: shRNA TRCN0000069177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080465.1-1528s1c1
DNA Barcode:
GAGACTTTGATTGGTAGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnn2 (140492)
Gene Description:
potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2
Transcript:
RefSeq NM_080465.1 (NON-CURRENT)
Match location:
Position 1528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTTTGATTGGTAGCATT
Hairpin Sequence:
5'-CCGG-GAGACTTTGATTGGTAGCATT-CTCGAG-AATGCTACCAATCAAAGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTTTGATTGGTAGCATTCTCGAGAATGCTACCAATCAAAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTTTGATTGGTAGCATTCTCGAGAATGCTACCAATCAAAGTCTC-3'

Other clones with same target sequence:

(none)