Construct: shRNA TRCN0000069193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080466.1-1182s1c1
DNA Barcode:
CCCTGGAGAGTACAAGTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnn3 (140493)
Gene Description:
potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Transcript:
RefSeq NM_080466.1 (NON-CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGAGAGTACAAGTTCTT
Hairpin Sequence:
5'-CCGG-CCCTGGAGAGTACAAGTTCTT-CTCGAG-AAGAACTTGTACTCTCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGAGAGTACAAGTTCTTCTCGAGAAGAACTTGTACTCTCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGAGAGTACAAGTTCTTCTCGAGAAGAACTTGTACTCTCCAGGG-3'

Other clones with same target sequence:

(none)