Construct: shRNA TRCN0000069210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007583.1-839s1c1
DNA Barcode:
CATCGGGATCATCGTGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacng2 (12300)
Gene Description:
calcium channel, voltage-dependent, gamma subunit 2
Transcript:
RefSeq NM_007583.1 (NON-CURRENT)
Match location:
Position 839 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGGGATCATCGTGTATAT
Hairpin Sequence:
5'-CCGG-CATCGGGATCATCGTGTATAT-CTCGAG-ATATACACGATGATCCCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGGGATCATCGTGTATATCTCGAGATATACACGATGATCCCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGGGATCATCGTGTATATCTCGAGATATACACGATGATCCCGATG-3'

Other clones with same target sequence:

(none)