Construct: shRNA TRCN0000069215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021275.2-2965s1c1
DNA Barcode:
CCTACCTTCTAATTTGCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcna4 (16492)
Gene Description:
potassium voltage-gated channel, shaker-related subfamily, member 4
Transcript:
RefSeq NM_021275.2 (NON-CURRENT)
Match location:
Position 2965 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCTTCTAATTTGCTCAA
Hairpin Sequence:
5'-CCGG-CCTACCTTCTAATTTGCTCAA-CTCGAG-TTGAGCAAATTAGAAGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCTTCTAATTTGCTCAACTCGAGTTGAGCAAATTAGAAGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCTTCTAATTTGCTCAACTCGAGTTGAGCAAATTAGAAGGTAGG-3'

Other clones with same target sequence:

(none)