Construct: shRNA TRCN0000069236
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_484912.1-449s1c1
- DNA Barcode:
- CTAGACTCCTTAGCAGCAGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC545361 (545361)
- Gene Description:
- similar to Voltage-dependent L-type calcium channel alpha-1S subunit (Voltage-gated calcium channel alpha subunit Cav1.1) (Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle)
- Transcript:
- RefSeq XM_484912.1 (NON-CURRENT)
- Match location:
- Position 449 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000069236)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTAGACTCCTTAGCAGCAGAT
- Hairpin Sequence:
-
5'-CCGG-CTAGACTCCTTAGCAGCAGAT-CTCGAG-ATCTGCTGCTAAGGAGTCTAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTAGACTCCTTAGCAGCAGATCTCGAGATCTGCTGCTAAGGAGTCTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTAGACTCCTTAGCAGCAGATCTCGAGATCTGCTGCTAAGGAGTCTAG-3'
Other clones with same target sequence:
(none)
