Construct: shRNA TRCN0000069244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145983.1-683s1c1
DNA Barcode:
GCTACTTCGATCCCTTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcna5 (16493)
Gene Description:
potassium voltage-gated channel, shaker-related subfamily, member 5
Transcript:
RefSeq NM_145983.1 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTTCGATCCCTTGAGAA
Hairpin Sequence:
5'-CCGG-GCTACTTCGATCCCTTGAGAA-CTCGAG-TTCTCAAGGGATCGAAGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTTCGATCCCTTGAGAACTCGAGTTCTCAAGGGATCGAAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTTCGATCCCTTGAGAACTCGAGTTCTCAAGGGATCGAAGTAGC-3'

Other clones with same target sequence:

(none)