Construct: shRNA TRCN0000069271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019431.1-786s1c1
DNA Barcode:
CCTGAAGATCACCGGAGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacng4 (54377)
Gene Description:
calcium channel, voltage-dependent, gamma subunit 4
Transcript:
RefSeq NM_019431.1 (NON-CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGATCACCGGAGCCAT
Hairpin Sequence:
5'-CCGG-CCTGAAGATCACCGGAGCCAT-CTCGAG-ATGGCTCCGGTGATCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGATCACCGGAGCCATCTCGAGATGGCTCCGGTGATCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGATCACCGGAGCCATCTCGAGATGGCTCCGGTGATCTTCAGG-3'

Other clones with same target sequence:

(none)