Construct: shRNA TRCN0000069282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145963.1-1301s1c1
DNA Barcode:
CCACAGCCCTAAGTCAAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnj14 (211480)
Gene Description:
potassium inwardly-rectifying channel, subfamily J, member 14
Transcript:
RefSeq NM_145963.1 (NON-CURRENT)
Match location:
Position 1301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCCCTAAGTCAAGTTT
Hairpin Sequence:
5'-CCGG-CCACAGCCCTAAGTCAAGTTT-CTCGAG-AAACTTGACTTAGGGCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCCCTAAGTCAAGTTTCTCGAGAAACTTGACTTAGGGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCCCTAAGTCAAGTTTCTCGAGAAACTTGACTTAGGGCTGTGG-3'

Other clones with same target sequence:

(none)