Construct: shRNA TRCN0000069289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133183.1-339s1c1
DNA Barcode:
CTGCACCTACTTCAAATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacng6 (54378)
Gene Description:
calcium channel, voltage-dependent, gamma subunit 6
Transcript:
RefSeq NM_133183.1 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCACCTACTTCAAATTCTT
Hairpin Sequence:
5'-CCGG-CTGCACCTACTTCAAATTCTT-CTCGAG-AAGAATTTGAAGTAGGTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCACCTACTTCAAATTCTTCTCGAGAAGAATTTGAAGTAGGTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCACCTACTTCAAATTCTTCTCGAGAAGAATTTGAAGTAGGTGCAG-3'

Other clones with same target sequence:

(none)