Construct: shRNA TRCN0000069396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_196271.2-405s1c1
DNA Barcode:
CCTTGAGCAGAATGAAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC269279 (269279)
Gene Description:
similar to sodium channel 25; sodium channel, type IX, alpha polypeptide; sodium channel, voltage-gated, type IX, alpha polypeptide
Transcript:
RefSeq XM_196271.2 (NON-CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGAGCAGAATGAAACATT
Hairpin Sequence:
5'-CCGG-CCTTGAGCAGAATGAAACATT-CTCGAG-AATGTTTCATTCTGCTCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGAGCAGAATGAAACATTCTCGAGAATGTTTCATTCTGCTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGAGCAGAATGAAACATTCTCGAGAATGTTTCATTCTGCTCAAGG-3'

Other clones with same target sequence:

(none)