Construct: shRNA TRCN0000069415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172650.2-394s1c1
DNA Barcode:
CGAGATGAAACTGGTGCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kctd3 (226823)
Gene Description:
potassium channel tetramerisation domain containing 3
Transcript:
RefSeq NM_172650.2 (CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGAAACTGGTGCTATA
Hairpin Sequence:
5'-CCGG-CGAGATGAAACTGGTGCTATA-CTCGAG-TATAGCACCAGTTTCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGAAACTGGTGCTATACTCGAGTATAGCACCAGTTTCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGAAACTGGTGCTATACTCGAGTATAGCACCAGTTTCATCTCG-3'

Other clones with same target sequence:

(none)