Construct: shRNA TRCN0000069432
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133190.1-165s1c1
- DNA Barcode:
- TCAGGTACTACTGACCACCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cacng8 (81905)
- Gene Description:
- calcium channel, voltage-dependent, gamma subunit 8
- Transcript:
- RefSeq NM_133190.1 (NON-CURRENT)
- Match location:
- Position 165 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000069432)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCAGGTACTACTGACCACCAT
- Hairpin Sequence:
-
5'-CCGG-TCAGGTACTACTGACCACCAT-CTCGAG-ATGGTGGTCAGTAGTACCTGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCAGGTACTACTGACCACCATCTCGAGATGGTGGTCAGTAGTACCTGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCAGGTACTACTGACCACCATCTCGAGATGGTGGTCAGTAGTACCTGA-3'
Other clones with same target sequence:
(none)
