Construct: shRNA TRCN0000069441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129704.4-309s1c1
DNA Barcode:
CGTTGATCAAATTCTTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnq5 (226922)
Gene Description:
potassium voltage-gated channel, subfamily Q, member 5
Transcript:
RefSeq XM_129704.4 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTGATCAAATTCTTGGAAA
Hairpin Sequence:
5'-CCGG-CGTTGATCAAATTCTTGGAAA-CTCGAG-TTTCCAAGAATTTGATCAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTGATCAAATTCTTGGAAACTCGAGTTTCCAAGAATTTGATCAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTGATCAAATTCTTGGAAACTCGAGTTTCCAAGAATTTGATCAACG-3'

Other clones with same target sequence:

(none)