Construct: shRNA TRCN0000069444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178675.3-677s1c1
DNA Barcode:
CCAGAGCTTTCTGAACTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc35f1 (215085)
Gene Description:
solute carrier family 35, member F1
Transcript:
RefSeq NM_178675.3 (NON-CURRENT)
Match location:
Position 677 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGCTTTCTGAACTACAT
Hairpin Sequence:
5'-CCGG-CCAGAGCTTTCTGAACTACAT-CTCGAG-ATGTAGTTCAGAAAGCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGCTTTCTGAACTACATCTCGAGATGTAGTTCAGAAAGCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGCTTTCTGAACTACATCTCGAGATGTAGTTCAGAAAGCTCTGG-3'

Other clones with same target sequence:

(none)