Construct: shRNA TRCN0000069458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008422.1-1394s1c1
DNA Barcode:
GCATGTACTATTCACTGGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnc3 (16504)
Gene Description:
potassium voltage gated channel, Shaw-related subfamily, member 3
Transcript:
RefSeq NM_008422.1 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTACTATTCACTGGCTA
Hairpin Sequence:
5'-CCGG-GCATGTACTATTCACTGGCTA-CTCGAG-TAGCCAGTGAATAGTACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTACTATTCACTGGCTACTCGAGTAGCCAGTGAATAGTACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTACTATTCACTGGCTACTCGAGTAGCCAGTGAATAGTACATGC-3'

Other clones with same target sequence:

(none)