Construct: shRNA TRCN0000069483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009198.2-1624s1c1
DNA Barcode:
GCCTAGACTAATGTCTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc17a1 (20504)
Gene Description:
solute carrier family 17 (sodium phosphate), member 1
Transcript:
RefSeq NM_009198.2 (NON-CURRENT)
Match location:
Position 1624 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGACTAATGTCTCTGAA
Hairpin Sequence:
5'-CCGG-GCCTAGACTAATGTCTCTGAA-CTCGAG-TTCAGAGACATTAGTCTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGACTAATGTCTCTGAACTCGAGTTCAGAGACATTAGTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGACTAATGTCTCTGAACTCGAGTTCAGAGACATTAGTCTAGGC-3'

Other clones with same target sequence:

(none)