Construct: shRNA TRCN0000069496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016691.2-1748s1c1
DNA Barcode:
CGGATGACTGTTTCTCTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clcn5 (12728)
Gene Description:
chloride channel, voltage-sensitive 5
Transcript:
RefSeq NM_016691.2 (NON-CURRENT)
Match location:
Position 1748 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGACTGTTTCTCTTGTT
Hairpin Sequence:
5'-CCGG-CGGATGACTGTTTCTCTTGTT-CTCGAG-AACAAGAGAAACAGTCATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGACTGTTTCTCTTGTTCTCGAGAACAAGAGAAACAGTCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGACTGTTTCTCTTGTTCTCGAGAACAAGAGAAACAGTCATCCG-3'

Other clones with same target sequence:

  1. TRCN0000352263