Construct: shRNA TRCN0000069545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011402.1-1628s1c1
DNA Barcode:
CGCAGTCTTCTATCTCATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc34a2 (20531)
Gene Description:
solute carrier family 34 (sodium phosphate), member 2
Transcript:
RefSeq NM_011402.1 (NON-CURRENT)
Match location:
Position 1628 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGTCTTCTATCTCATCTT
Hairpin Sequence:
5'-CCGG-CGCAGTCTTCTATCTCATCTT-CTCGAG-AAGATGAGATAGAAGACTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGTCTTCTATCTCATCTTCTCGAGAAGATGAGATAGAAGACTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGTCTTCTATCTCATCTTCTCGAGAAGATGAGATAGAAGACTGCG-3'

Other clones with same target sequence:

(none)