Construct: shRNA TRCN0000069568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020269.3-221s1c1
DNA Barcode:
CGACCTTCATTGACATGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnj10 (16513)
Gene Description:
potassium inwardly-rectifying channel, subfamily J, member 10
Transcript:
RefSeq NM_020269.3 (NON-CURRENT)
Match location:
Position 221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCTTCATTGACATGCAAT
Hairpin Sequence:
5'-CCGG-CGACCTTCATTGACATGCAAT-CTCGAG-ATTGCATGTCAATGAAGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCTTCATTGACATGCAATCTCGAGATTGCATGTCAATGAAGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCTTCATTGACATGCAATCTCGAGATTGCATGTCAATGAAGGTCG-3'

Other clones with same target sequence:

(none)