Construct: shRNA TRCN0000069648
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_357563.1-282s1c1
- DNA Barcode:
- CCAGTTGATTCTTGGTGTGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC384313 (384313)
- Gene Description:
- similar to voltage-gated calcium channel alpha2/delta subunit, alpha2a isoform
- Transcript:
- RefSeq XM_357563.1 (NON-CURRENT)
- Match location:
- Position 282 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000069648)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGTTGATTCTTGGTGTGAT
- Hairpin Sequence:
-
5'-CCGG-CCAGTTGATTCTTGGTGTGAT-CTCGAG-ATCACACCAAGAATCAACTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGTTGATTCTTGGTGTGATCTCGAGATCACACCAAGAATCAACTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGTTGATTCTTGGTGTGATCTCGAGATCACACCAAGAATCAACTGG-3'
Other clones with same target sequence:
(none)
