Construct: shRNA TRCN0000069649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357563.1-330s1c1
DNA Barcode:
CAAGAGATTGACACCACGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC384313 (384313)
Gene Description:
similar to voltage-gated calcium channel alpha2/delta subunit, alpha2a isoform
Transcript:
RefSeq XM_357563.1 (NON-CURRENT)
Match location:
Position 330 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGATTGACACCACGTTT
Hairpin Sequence:
5'-CCGG-CAAGAGATTGACACCACGTTT-CTCGAG-AAACGTGGTGTCAATCTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGATTGACACCACGTTTCTCGAGAAACGTGGTGTCAATCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGATTGACACCACGTTTCTCGAGAAACGTGGTGTCAATCTCTTG-3'

Other clones with same target sequence:

(none)