Construct: shRNA TRCN0000069656
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_136435.2-475s1c1
- DNA Barcode:
- GCCTTTGAGCAGACCAAGTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC240711 (240711)
- Gene Description:
- similar to Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (P-Rex1 protein)
- Transcript:
- RefSeq XM_136435.2 (NON-CURRENT)
- Match location:
- Position 475 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000069656)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTTTGAGCAGACCAAGTAT
- Hairpin Sequence:
-
5'-CCGG-GCCTTTGAGCAGACCAAGTAT-CTCGAG-ATACTTGGTCTGCTCAAAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTTTGAGCAGACCAAGTATCTCGAGATACTTGGTCTGCTCAAAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTTTGAGCAGACCAAGTATCTCGAGATACTTGGTCTGCTCAAAGGC-3'
Other clones with same target sequence:
(none)
