Construct: shRNA TRCN0000069668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175519.3-1282s1c1
DNA Barcode:
CGCGACAAAGTCACAGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kctd8 (243043)
Gene Description:
potassium channel tetramerisation domain containing 8
Transcript:
RefSeq NM_175519.3 (NON-CURRENT)
Match location:
Position 1282 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGACAAAGTCACAGACAAA
Hairpin Sequence:
5'-CCGG-CGCGACAAAGTCACAGACAAA-CTCGAG-TTTGTCTGTGACTTTGTCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGACAAAGTCACAGACAAACTCGAGTTTGTCTGTGACTTTGTCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGACAAAGTCACAGACAAACTCGAGTTTGTCTGTGACTTTGTCGCG-3'

Other clones with same target sequence:

(none)