Construct: shRNA TRCN0000069718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178934.2-960s1c1
DNA Barcode:
GCAGCCAAACATACTATTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc2a12 (353169)
Gene Description:
solute carrier family 2 (facilitated glucose transporter), member 12
Transcript:
RefSeq NM_178934.2 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCAAACATACTATTCTA
Hairpin Sequence:
5'-CCGG-GCAGCCAAACATACTATTCTA-CTCGAG-TAGAATAGTATGTTTGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCAAACATACTATTCTACTCGAGTAGAATAGTATGTTTGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCAAACATACTATTCTACTCGAGTAGAATAGTATGTTTGGCTGC-3'

Other clones with same target sequence:

(none)