Construct: shRNA TRCN0000069733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008426.1-1345s1c1
DNA Barcode:
CCCATGAAACTCCAACGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnj3 (16519)
Gene Description:
potassium inwardly-rectifying channel, subfamily J, member 3
Transcript:
RefSeq NM_008426.1 (NON-CURRENT)
Match location:
Position 1345 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGAAACTCCAACGAATA
Hairpin Sequence:
5'-CCGG-CCCATGAAACTCCAACGAATA-CTCGAG-TATTCGTTGGAGTTTCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGAAACTCCAACGAATACTCGAGTATTCGTTGGAGTTTCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGAAACTCCAACGAATACTCGAGTATTCGTTGGAGTTTCATGGG-3'

Other clones with same target sequence:

(none)