Construct: shRNA TRCN0000069752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_133347.2-1385s1c1
DNA Barcode:
GCAGTGACTACTTCGACACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381861 (381861)
Gene Description:
similar to ryanodine receptor 1 (skeletal); sarcoplasmic reticulum calcium release channel
Transcript:
RefSeq XM_133347.2 (NON-CURRENT)
Match location:
Position 1385 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGACTACTTCGACACAA
Hairpin Sequence:
5'-CCGG-GCAGTGACTACTTCGACACAA-CTCGAG-TTGTGTCGAAGTAGTCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGACTACTTCGACACAACTCGAGTTGTGTCGAAGTAGTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGACTACTTCGACACAACTCGAGTTGTGTCGAAGTAGTCACTGC-3'

Other clones with same target sequence:

(none)