Construct: shRNA TRCN0000069846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172892.1-1413s1c1
DNA Barcode:
CCAAGAACTGAACAAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc13a4 (243755)
Gene Description:
solute carrier family 13 (sodium/sulfate symporters), member 4
Transcript:
RefSeq NM_172892.1 (NON-CURRENT)
Match location:
Position 1413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAACTGAACAAGAAGAA
Hairpin Sequence:
5'-CCGG-CCAAGAACTGAACAAGAAGAA-CTCGAG-TTCTTCTTGTTCAGTTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAACTGAACAAGAAGAACTCGAGTTCTTCTTGTTCAGTTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAACTGAACAAGAAGAACTCGAGTTCTTCTTGTTCAGTTCTTGG-3'

Other clones with same target sequence:

(none)