Construct: shRNA TRCN0000069875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_140499.3-600s1c1
DNA Barcode:
CCACACCTTCTCGCGCTCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcng2 (240444)
Gene Description:
potassium voltage-gated channel, subfamily G, member 2
Transcript:
RefSeq XM_140499.3 (NON-CURRENT)
Match location:
Position 600 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACCTTCTCGCGCTCCTA
Hairpin Sequence:
5'-CCGG-CCACACCTTCTCGCGCTCCTA-CTCGAG-TAGGAGCGCGAGAAGGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACCTTCTCGCGCTCCTACTCGAGTAGGAGCGCGAGAAGGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACCTTCTCGCGCTCCTACTCGAGTAGGAGCGCGAGAAGGTGTGG-3'

Other clones with same target sequence:

(none)