Construct: shRNA TRCN0000069893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053195.1-3233s1c1
DNA Barcode:
CGCTGAATTATTATGCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc24a3 (94249)
Gene Description:
solute carrier family 24 (sodium/potassium/calcium exchanger), member 3
Transcript:
RefSeq NM_053195.1 (NON-CURRENT)
Match location:
Position 3233 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGAATTATTATGCAGAAT
Hairpin Sequence:
5'-CCGG-CGCTGAATTATTATGCAGAAT-CTCGAG-ATTCTGCATAATAATTCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGAATTATTATGCAGAATCTCGAGATTCTGCATAATAATTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGAATTATTATGCAGAATCTCGAGATTCTGCATAATAATTCAGCG-3'

Other clones with same target sequence:

(none)