Construct: shRNA TRCN0000069953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021353.1-1736s1c1
DNA Barcode:
CCAATCTACTTCGCAAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc26a3 (13487)
Gene Description:
solute carrier family 26, member 3
Transcript:
RefSeq NM_021353.1 (NON-CURRENT)
Match location:
Position 1736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCTACTTCGCAAACATT
Hairpin Sequence:
5'-CCGG-CCAATCTACTTCGCAAACATT-CTCGAG-AATGTTTGCGAAGTAGATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCTACTTCGCAAACATTCTCGAGAATGTTTGCGAAGTAGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCTACTTCGCAAACATTCTCGAGAATGTTTGCGAAGTAGATTGG-3'

Other clones with same target sequence:

(none)