Construct: shRNA TRCN0000069961
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_355194.1-126s1c1
- DNA Barcode:
- GCAGTCAGAAGCTGAGCTTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC381252 (381252)
- Gene Description:
- similar to Potassium voltage-gated channel subfamily KQT member 5 (Potassium channel alpha subunit KvLQT5) (KQT-like 5)
- Transcript:
- RefSeq XM_355194.1 (NON-CURRENT)
- Match location:
- Position 126 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000069961)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTCAGAAGCTGAGCTTTA
- Hairpin Sequence:
-
5'-CCGG-GCAGTCAGAAGCTGAGCTTTA-CTCGAG-TAAAGCTCAGCTTCTGACTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTCAGAAGCTGAGCTTTACTCGAGTAAAGCTCAGCTTCTGACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTCAGAAGCTGAGCTTTACTCGAGTAAAGCTCAGCTTCTGACTGC-3'
Other clones with same target sequence:
(none)
