Construct: shRNA TRCN0000069962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355194.1-64s1c1
DNA Barcode:
GCCACATCTGAAGGCCTTGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381252 (381252)
Gene Description:
similar to Potassium voltage-gated channel subfamily KQT member 5 (Potassium channel alpha subunit KvLQT5) (KQT-like 5)
Transcript:
RefSeq XM_355194.1 (NON-CURRENT)
Match location:
Position 64 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATCTGAAGGCCTTGCA
Hairpin Sequence:
5'-CCGG-GCCACATCTGAAGGCCTTGCA-CTCGAG-TGCAAGGCCTTCAGATGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATCTGAAGGCCTTGCACTCGAGTGCAAGGCCTTCAGATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATCTGAAGGCCTTGCACTCGAGTGCAAGGCCTTCAGATGTGGC-3'

Other clones with same target sequence:

(none)