Construct: shRNA TRCN0000069974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172152.1-1765s1c1
DNA Barcode:
CCTGTGCTTCTCCATAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc24a4 (238384)
Gene Description:
solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Transcript:
RefSeq NM_172152.1 (NON-CURRENT)
Match location:
Position 1765 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGCTTCTCCATAATGAT
Hairpin Sequence:
5'-CCGG-CCTGTGCTTCTCCATAATGAT-CTCGAG-ATCATTATGGAGAAGCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGCTTCTCCATAATGATCTCGAGATCATTATGGAGAAGCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGCTTCTCCATAATGATCTCGAGATCATTATGGAGAAGCACAGG-3'

Other clones with same target sequence:

(none)