Construct: shRNA TRCN0000070009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021542.2-556s1c1
DNA Barcode:
CGGGAACCAGACTTTCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnk5 (16529)
Gene Description:
potassium channel, subfamily K, member 5
Transcript:
RefSeq NM_021542.2 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAACCAGACTTTCAACAA
Hairpin Sequence:
5'-CCGG-CGGGAACCAGACTTTCAACAA-CTCGAG-TTGTTGAAAGTCTGGTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAACCAGACTTTCAACAACTCGAGTTGTTGAAAGTCTGGTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAACCAGACTTTCAACAACTCGAGTTGTTGAAAGTCTGGTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000316947