Construct: shRNA TRCN0000070053
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_357550.1-295s1c1
- DNA Barcode:
- GCACTTCATTTGTATTCCCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC384294 (384294)
- Gene Description:
- similar to Potassium channel subfamily K member 3 (Acid-sensitive potassium channel protein TASK-1) (TWIK-related acid-sensitive K+ channel 1) (Two pore potassium channel KT3.1)
- Transcript:
- RefSeq XM_357550.1 (NON-CURRENT)
- Match location:
- Position 295 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000070053)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACTTCATTTGTATTCCCTT
- Hairpin Sequence:
-
5'-CCGG-GCACTTCATTTGTATTCCCTT-CTCGAG-AAGGGAATACAAATGAAGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACTTCATTTGTATTCCCTTCTCGAGAAGGGAATACAAATGAAGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACTTCATTTGTATTCCCTTCTCGAGAAGGGAATACAAATGAAGTGC-3'
Other clones with same target sequence:
(none)
