Construct: shRNA TRCN0000070054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357550.1-96s1c1
DNA Barcode:
GCCGGAGATGATCGAGCGGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC384294 (384294)
Gene Description:
similar to Potassium channel subfamily K member 3 (Acid-sensitive potassium channel protein TASK-1) (TWIK-related acid-sensitive K+ channel 1) (Two pore potassium channel KT3.1)
Transcript:
RefSeq XM_357550.1 (NON-CURRENT)
Match location:
Position 96 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGAGATGATCGAGCGGCA
Hairpin Sequence:
5'-CCGG-GCCGGAGATGATCGAGCGGCA-CTCGAG-TGCCGCTCGATCATCTCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGAGATGATCGAGCGGCACTCGAGTGCCGCTCGATCATCTCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGAGATGATCGAGCGGCACTCGAGTGCCGCTCGATCATCTCCGGC-3'

Other clones with same target sequence:

(none)