Construct: shRNA TRCN0000070060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011644.1-287s1c1
DNA Barcode:
CCTGGCTGACTAATCCTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trpc2 (22064)
Gene Description:
transient receptor potential cation channel, subfamily C, member 2
Transcript:
RefSeq NM_011644.1 (NON-CURRENT)
Match location:
Position 287 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCTGACTAATCCTTCTA
Hairpin Sequence:
5'-CCGG-CCTGGCTGACTAATCCTTCTA-CTCGAG-TAGAAGGATTAGTCAGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCTGACTAATCCTTCTACTCGAGTAGAAGGATTAGTCAGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCTGACTAATCCTTCTACTCGAGTAGAAGGATTAGTCAGCCAGG-3'

Other clones with same target sequence:

(none)