Construct: shRNA TRCN0000070071

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172523.1-604s1c1
DNA Barcode:
GCTGGATTCTGCATTATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc18a2 (214084)
Gene Description:
solute carrier family 18 (vesicular monoamine), member 2
Transcript:
RefSeq NM_172523.1 (NON-CURRENT)
Match location:
Position 604 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070071)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATTCTGCATTATGTTT
Hairpin Sequence:
5'-CCGG-GCTGGATTCTGCATTATGTTT-CTCGAG-AAACATAATGCAGAATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATTCTGCATTATGTTTCTCGAGAAACATAATGCAGAATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATTCTGCATTATGTTTCTCGAGAAACATAATGCAGAATCCAGC-3'

Other clones with same target sequence:

(none)