Construct: shRNA TRCN0000070079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010020.2-1657s1c1
DNA Barcode:
CCAGCAATTCAGTGATGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a3 (13162)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
Transcript:
RefSeq NM_010020.2 (NON-CURRENT)
Match location:
Position 1657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAATTCAGTGATGACAT
Hairpin Sequence:
5'-CCGG-CCAGCAATTCAGTGATGACAT-CTCGAG-ATGTCATCACTGAATTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAATTCAGTGATGACATCTCGAGATGTCATCACTGAATTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAATTCAGTGATGACATCTCGAGATGTCATCACTGAATTGCTGG-3'

Other clones with same target sequence:

(none)