Construct: shRNA TRCN0000070099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020258.2-696s1c1
DNA Barcode:
GCCTGGCATTATCACTGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc37a2 (56857)
Gene Description:
solute carrier family 37 (glycerol-3-phosphate transporter), member 2
Transcript:
RefSeq NM_020258.2 (NON-CURRENT)
Match location:
Position 696 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGCATTATCACTGCTAT
Hairpin Sequence:
5'-CCGG-GCCTGGCATTATCACTGCTAT-CTCGAG-ATAGCAGTGATAATGCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGCATTATCACTGCTATCTCGAGATAGCAGTGATAATGCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGCATTATCACTGCTATCTCGAGATAGCAGTGATAATGCCAGGC-3'

Other clones with same target sequence:

(none)