Construct: shRNA TRCN0000070129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008767.1-617s1c1
DNA Barcode:
CCTCTGCTTTGGCATTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc22a18 (18400)
Gene Description:
solute carrier family 22 (organic cation transporter), member 18
Transcript:
RefSeq NM_008767.1 (NON-CURRENT)
Match location:
Position 617 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGCTTTGGCATTGGAAT
Hairpin Sequence:
5'-CCGG-CCTCTGCTTTGGCATTGGAAT-CTCGAG-ATTCCAATGCCAAAGCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGCTTTGGCATTGGAATCTCGAGATTCCAATGCCAAAGCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGCTTTGGCATTGGAATCTCGAGATTCCAATGCCAAAGCAGAGG-3'

Other clones with same target sequence:

(none)