Construct: shRNA TRCN0000070165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145390.1-1396s1c1
DNA Barcode:
CCAGTGCCTGTCAGATAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnpo2 (212999)
Gene Description:
transportin 2 (importin 3, karyopherin beta 2b)
Transcript:
RefSeq NM_145390.1 (NON-CURRENT)
Match location:
Position 1396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGCCTGTCAGATAAGAA
Hairpin Sequence:
5'-CCGG-CCAGTGCCTGTCAGATAAGAA-CTCGAG-TTCTTATCTGACAGGCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGCCTGTCAGATAAGAACTCGAGTTCTTATCTGACAGGCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGCCTGTCAGATAAGAACTCGAGTTCTTATCTGACAGGCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000324596