Construct: shRNA TRCN0000070215
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_009727.1-1008s1c1
- DNA Barcode:
- CGCTAGTAACTTTGGACTGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Atp8a1 (11980)
- Gene Description:
- ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
- Transcript:
- RefSeq NM_009727.1 (NON-CURRENT)
- Match location:
- Position 1008 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000070215)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCTAGTAACTTTGGACTGAA
- Hairpin Sequence:
-
5'-CCGG-CGCTAGTAACTTTGGACTGAA-CTCGAG-TTCAGTCCAAAGTTACTAGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCTAGTAACTTTGGACTGAACTCGAGTTCAGTCCAAAGTTACTAGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCTAGTAACTTTGGACTGAACTCGAGTTCAGTCCAAAGTTACTAGCG-3'
Other clones with same target sequence:
(none)
