Construct: shRNA TRCN0000070241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133661.1-696s1c1
DNA Barcode:
CGAGTCTTATTTGAACATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a12 (14411)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Transcript:
RefSeq NM_133661.1 (NON-CURRENT)
Match location:
Position 696 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTCTTATTTGAACATCTA
Hairpin Sequence:
5'-CCGG-CGAGTCTTATTTGAACATCTA-CTCGAG-TAGATGTTCAAATAAGACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTCTTATTTGAACATCTACTCGAGTAGATGTTCAAATAAGACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTCTTATTTGAACATCTACTCGAGTAGATGTTCAAATAAGACTCG-3'

Other clones with same target sequence:

(none)