Construct: shRNA TRCN0000070273

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021551.1-1791s1c1
DNA Barcode:
GAAAGATAGACAGGAAGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc22a17 (59049)
Gene Description:
solute carrier family 22 (organic cation transporter), member 17
Transcript:
RefSeq NM_021551.1 (NON-CURRENT)
Match location:
Position 1791 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070273)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGATAGACAGGAAGGCAA
Hairpin Sequence:
5'-CCGG-GAAAGATAGACAGGAAGGCAA-CTCGAG-TTGCCTTCCTGTCTATCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGATAGACAGGAAGGCAACTCGAGTTGCCTTCCTGTCTATCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGATAGACAGGAAGGCAACTCGAGTTGCCTTCCTGTCTATCTTTC-3'

Other clones with same target sequence:

(none)