Construct: shRNA TRCN0000070289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174870.2-638s1c1
DNA Barcode:
GCTGGGTTACAACCCATCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc26a1 (231583)
Gene Description:
solute carrier family 26 (sulfate transporter), member 1
Transcript:
RefSeq NM_174870.2 (NON-CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGTTACAACCCATCTAT
Hairpin Sequence:
5'-CCGG-GCTGGGTTACAACCCATCTAT-CTCGAG-ATAGATGGGTTGTAACCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGTTACAACCCATCTATCTCGAGATAGATGGGTTGTAACCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGTTACAACCCATCTATCTCGAGATAGATGGGTTGTAACCCAGC-3'

Other clones with same target sequence:

(none)