Construct: shRNA TRCN0000070295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_125547.2-171s1c1
DNA Barcode:
CCTGTATCTGATATGTGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc22a16 (70840)
Gene Description:
solute carrier family 22 (organic cation transporter), member 16
Transcript:
RefSeq XM_125547.2 (NON-CURRENT)
Match location:
Position 171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTATCTGATATGTGCCTA
Hairpin Sequence:
5'-CCGG-CCTGTATCTGATATGTGCCTA-CTCGAG-TAGGCACATATCAGATACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTATCTGATATGTGCCTACTCGAGTAGGCACATATCAGATACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTATCTGATATGTGCCTACTCGAGTAGGCACATATCAGATACAGG-3'

Other clones with same target sequence:

(none)