Construct: shRNA TRCN0000070313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030683.1-420s1c1
DNA Barcode:
GCCTCTTTCCAGCAGATACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc14a2 (27411)
Gene Description:
solute carrier family 14 (urea transporter), member 2
Transcript:
RefSeq NM_030683.1 (NON-CURRENT)
Match location:
Position 420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTTCCAGCAGATACAA
Hairpin Sequence:
5'-CCGG-GCCTCTTTCCAGCAGATACAA-CTCGAG-TTGTATCTGCTGGAAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTTCCAGCAGATACAACTCGAGTTGTATCTGCTGGAAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTTCCAGCAGATACAACTCGAGTTGTATCTGCTGGAAAGAGGC-3'

Other clones with same target sequence:

(none)